neuroscience 2012

Nephew's David's paper is now available online.

Nicaise, Charles, Hala, Tamara J., Frank, David M., Parker,

Jessica L., Authelet, Mich`ele, Leroy, Karelle, Brion, Jean-Pierre, Wright, Megan C.,

Lepore, Angelo C., Phrenic motor neuron degeneration compromises phrenic axonal circuitry

and diaphragm activity in a unilateral cervical contusion model of spinal cord

injury, Experimental Neurology (2012), doi: 10.1016/j.expneurol.2012.03.007

http://www.sciencedirect.com/science/article/pii/S0014488612001057

Abstract

Respiratory dysfunction is the leading cause of morbidity and mortality following traumatic spinal cord injury (SCI). Injuries targeting mid-cervical spinal cord regions affect the phrenic motor neuron pool that innervates the diaphragm, the primary respiratory muscle of inspiration. Contusion-type injury in the cervical spinal cord is one of the most common forms of human SCI; however, few studies have evaluated mid-cervical contusion in animal models or characterized consequent histopathological and functional effects of degeneration of phrenic motor neuron-diaphragm circuitry. In an attempt to target the phrenic motor neuron pool, two unilateral contusion injury paradigms were tested, a single injury at level C4 and a double injury both at levels C3 and C4, and animals were followed for up to 6 weeks post-injury. Both unilateral cervical injury paradigms are reproducible with no mortality or need for breathing assistance, and are accompanied by phrenic motor neuron loss, phrenic nerve axon degeneration, diaphragm atrophy, denervation and subsequent partial reinnervation at the diaphragm neuromuscular junction, changes in spontaneous diaphragm EMG recordings, and reduction in phrenic nerve compound muscle action potential amplitude. These findings demonstrate significant and chronically-persistent respiratory compromise following mid-cervical SCI due to phrenic motor neuron degeneration. These injury paradigms and accompanying analyses provide important tools both for understanding mechanisms of phrenic motor neuron and diaphragm pathology following SCI and for evaluating therapeutic strategies in clinically-relevant cervical SCI models/

Intact neuromuscular junction in phrenic muscle, characterized by: complete overlap of the pre-synaptic axon and pre-synaptic vesicles with post-synaptic acetylcholine receptors.